Coding

Part:BBa_K108000:Design

Designed by: Qi Liu   Group: iGEM08_Tsinghua   (2008-10-03)

MutS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1164
    Illegal AgeI site found at 1837
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1291
    Illegal SapI.rc site found at 1585
    Illegal SapI.rc site found at 2547


Design Notes

PCR using primers with biobrick prefix and surfix, and then cloned into pSB1AC3.

Source

MutS was cloned from E.coli K12 DH10B genome (reference 1).

References

1. NCBI.